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recombinant human mmp-2 (pro-enzyme)  (AnaSpec)

 
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    Structured Review

    AnaSpec recombinant human mmp-2 (pro-enzyme)
    Key resources
    Recombinant Human Mmp 2 (Pro Enzyme), supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human mmp-2 (pro-enzyme)/product/AnaSpec
    Average 90 stars, based on 1 article reviews
    recombinant human mmp-2 (pro-enzyme) - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging"

    Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

    Journal: eNeuro

    doi: 10.1523/ENEURO.0031-23.2023

    Key resources
    Figure Legend Snippet: Key resources

    Techniques Used: Recombinant, Diagnostic Assay, Plasmid Preparation, Staining, Extraction, Sterility, Saline, Construct, Electron Microscopy, Expressing, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, Multiplex Assay, Transfection, Sequencing, Software, Control



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    Image Search Results


    Key resources

    Journal: eNeuro

    Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

    doi: 10.1523/ENEURO.0031-23.2023

    Figure Lengend Snippet: Key resources

    Article Snippet: Peptide, recombinant protein , Recombinant Human MMP-2 (pro-enzyme) , AnaSpec , Catalog #AS-72005 , Pro-enzyme Hu-pro-MMP2.2.

    Techniques: Recombinant, Diagnostic Assay, Plasmid Preparation, Staining, Extraction, Sterility, Saline, Construct, Electron Microscopy, Expressing, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, Multiplex Assay, Transfection, Sequencing, Software, Control

    Key resources

    Journal: eNeuro

    Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

    doi: 10.1523/ENEURO.0031-23.2023

    Figure Lengend Snippet: Key resources

    Article Snippet: The ability of the protein constructs to inhibit MMP2 was analyzed using the SensoLyte 520 MMP-2 Assay kit (AS-71151, AnaSpec) and recombinant human MMP-2 pro-enzyme (Hu-pro-MMP2.0, 902-MP-010, R&D Systems; Hu-pro-MMP2.2, AS-72005, AnaSpec).

    Techniques: Recombinant, Diagnostic Assay, Plasmid Preparation, Staining, Extraction, Sterility, Saline, Construct, Electron Microscopy, Expressing, Enzyme-linked Immunosorbent Assay, SYBR Green Assay, Multiplex Assay, Transfection, Sequencing, Software, Control

    The TIMP2 constructs had distinct MMP inhibitory profiles

    Journal: eNeuro

    Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

    doi: 10.1523/ENEURO.0031-23.2023

    Figure Lengend Snippet: The TIMP2 constructs had distinct MMP inhibitory profiles

    Article Snippet: The ability of the protein constructs to inhibit MMP2 was analyzed using the SensoLyte 520 MMP-2 Assay kit (AS-71151, AnaSpec) and recombinant human MMP-2 pro-enzyme (Hu-pro-MMP2.0, 902-MP-010, R&D Systems; Hu-pro-MMP2.2, AS-72005, AnaSpec).

    Techniques: Construct, Inhibition

    The alanine insertion into TIMP2 prevented MMP inhibitory activity at biologically relevant concentrations

    Journal: eNeuro

    Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

    doi: 10.1523/ENEURO.0031-23.2023

    Figure Lengend Snippet: The alanine insertion into TIMP2 prevented MMP inhibitory activity at biologically relevant concentrations

    Article Snippet: The ability of the protein constructs to inhibit MMP2 was analyzed using the SensoLyte 520 MMP-2 Assay kit (AS-71151, AnaSpec) and recombinant human MMP-2 pro-enzyme (Hu-pro-MMP2.0, 902-MP-010, R&D Systems; Hu-pro-MMP2.2, AS-72005, AnaSpec).

    Techniques: Activity Assay

    Characterization of TIMP2-MMP binding of the TIMP2 constructs

    Journal: eNeuro

    Article Title: Noncanonical Activity of Tissue Inhibitor of Metalloproteinases 2 (TIMP2) Improves Cognition and Synapse Density in Aging

    doi: 10.1523/ENEURO.0031-23.2023

    Figure Lengend Snippet: Characterization of TIMP2-MMP binding of the TIMP2 constructs

    Article Snippet: The ability of the protein constructs to inhibit MMP2 was analyzed using the SensoLyte 520 MMP-2 Assay kit (AS-71151, AnaSpec) and recombinant human MMP-2 pro-enzyme (Hu-pro-MMP2.0, 902-MP-010, R&D Systems; Hu-pro-MMP2.2, AS-72005, AnaSpec).

    Techniques: Binding Assay

    MMP-2 digestion of ELP-mmpL-CPP. ( A ) ELP-mmpL-CPP-rhodamine and ELP-CPP-rhodamine were incubated with MMP-2 for 4 h in ZnCl 2 buffer (pH 7). ( B ) MMP-2 incubation of the constructs produced ELP-CPP-Dox (63 kDa, upper band) and CPP-rhodamine (3 kDa, lower band). Left panel: silver-stained gel; Right panel: fluorescence-scanned gel. Dox: 6-maleimidocaproyl amide derivative of doxorubicin; mmpL: MMP substrate.

    Journal: International Journal of Molecular Sciences

    Article Title: Cell-Penetrating Doxorubicin Released from Elastin-Like Polypeptide Kills Doxorubicin-Resistant Cancer Cells in In Vitro Study

    doi: 10.3390/ijms22031126

    Figure Lengend Snippet: MMP-2 digestion of ELP-mmpL-CPP. ( A ) ELP-mmpL-CPP-rhodamine and ELP-CPP-rhodamine were incubated with MMP-2 for 4 h in ZnCl 2 buffer (pH 7). ( B ) MMP-2 incubation of the constructs produced ELP-CPP-Dox (63 kDa, upper band) and CPP-rhodamine (3 kDa, lower band). Left panel: silver-stained gel; Right panel: fluorescence-scanned gel. Dox: 6-maleimidocaproyl amide derivative of doxorubicin; mmpL: MMP substrate.

    Article Snippet: Recombinant human pro-MMP-2 (Enzo life science) was activated with 2.5 mM 4-aminophenylmercuric acetate at 37 °C for 2 h. Then, 1 µg of each ELP construct was incubated with the pre-activated MMP-2 (10 pmol) for 4 h in a reaction buffer (50 mM Tris, 200 mM NaCl, 10 mM CaCl 2 , and 10 mM ZnCl 2 , pH 7.5).

    Techniques: Incubation, Construct, Produced, Staining, Fluorescence